Fatty acid composition, antimicrobial and antioxidant activity of cassia glauca seed extract

Abstract

The objective of the study was to investigate phytochemical analysis, antimicrobial and antioxidant activity of seeds of different extracts of Cassia glauca as well as their fatty acid composition of petroleum ether extract. All extract were tested for presence of phytoconstituents i.e., alkaloid, carbohydrate, sterols, proteins, amino acids, saponin, and phenolic compounds in different extracts. Antimicrobial activity was done by well diffusion method at a concentration of 200 mg/ml. Antioxidant activity was done by DPPH method at a different concentration 50 μg/ml to 500 μg/ml. GCMS Analysis was done for petroleum ether extract with the help of PERKIN ELMER CLARUS-500 model coupled with CLARUS-500 Mass spectrometer. Phytochemical analysis showed the presence of alkaloid, carbohydrate, sterols, proteins, amino acids, saponin, and phenolic compounds in different extracts. Methanol extract was the richest extract for tested phytoconstituents. Different fatty acids were present in petroleum ether extract which analysed by GCMS analysis. Maximum antimicrobial activity of seeds of Cassia glauca showed in acetone and methanol extract in comparison to standard drug. From antioxidant studies, acetone extract showed maximum antioxidant activity (76.11 %) than other extract in comparison to standard drug ascorbic acid. From above studies it could be concluded that methanol and acetone extract showed maximum antimicrobial and antioxidant activity.